M3-PAmcherry1
(Plasmid #114189)

• Purpose: studying clustering of M3 receptors by superresolution microscopy (PALM)
• Depositing Lab: Aleksandra Radenovic
• Depositing Organization: Swiss Federal Institute of Technology (EPFL)
  Located in Switzerland
• Publication: Radenovic Lab plasmids (unpublished)
• Citations: 1 Article

Backbone

• Vector backbone: pPA-mCherry N1
• Backbone size w/o insert (bp): 4727
• Modifications to backbone: pcDNA3.1-M3 receptor (wt) was purchased from cDNA.org. They amplified M3 by PCR from human whole brain cDNA (Clontech).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Muscarin acetylcholine receptor 3
• Alt name: M3
• Alt name: CHRM3
• Species: H. sapiens (human)
• Insert Size (bp): 1790
• Entrez Gene: CHRM3 (a.k.a. EGBRS, HM3, PBS, m3AChR)
• Promoter: SV40
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • PAmCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 114189-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 114189-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.