This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

M3-PAmcherry1
(Plasmid #114189)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114189 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPA-mCherry N1
  • Backbone size w/o insert (bp) 4727
  • Modifications to backbone
    pcDNA3.1-M3 receptor (wt) was purchased from cDNA.org. They amplified M3 by PCR from human whole brain cDNA (Clontech).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Muscarin acetylcholine receptor 3
  • Alt name
    M3
  • Alt name
    CHRM3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1790
  • Entrez Gene
    CHRM3 (a.k.a. EGBRS, HM3, PBS)
  • Promoter SV40
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • PAmCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M3-PAmcherry1 was a gift from Aleksandra Radenovic (Addgene plasmid # 114189 ; http://n2t.net/addgene:114189 ; RRID:Addgene_114189)