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(Bacterial strain #114268)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Bacterial Strain 114268 Bacteria in agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    The strain is resistant to 25 µg/ml zeocin selection. Genotype:F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) nupG arsB::M2.NmeMC58II-ble(ZeoR)
  • Copy number


  • Gene/Insert name

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Supplemental Documents for strain verification protocols and data.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DH10B-M2.NmeMC58II was a gift from Christopher A. O'Callaghan (Addgene plasmid # 114268)
  • For your References section:

    MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. Lin D, O'Callaghan CA. Nucleic Acids Res. 2018 Jul 9. pii: 5050631. doi: 10.1093/nar/gky596. 10.1093/nar/gky596 PubMed 29986052