PurposeThe strain expresses M.XmnI methylase and zeocin selection marker from the arsB locus.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||114269||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth instructionsThe strain is resistant to 25 µg/ml zeocin selection. Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) nupG arsB::M.XmnI-ble(ZeoR)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See Supplemental Documents for strain verification protocols and data.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DH10B-M.XmnI was a gift from Christopher A. O'Callaghan (Addgene plasmid # 114269)
For your References section:MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. Lin D, O'Callaghan CA. Nucleic Acids Res. 2018 Jul 9. pii: 5050631. doi: 10.1093/nar/gky596. 10.1093/nar/gky596 PubMed 29986052