pMLS253
(Plasmid
#114271)
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PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)
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Depositing Lab
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Depositing OrganizationUniversity of Utah
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 114271-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 114271-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDONR-221
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 4800
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Vector typeCre/Lox, CRISPR
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Selectable markersUnc-119
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCbr-Unc-119
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SpeciesCaenorhabditis briggsae
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Insert Size (bp)2279
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Mutation*please see depositor comment below
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: the Cbr-unc-119 rescue cassette contains 3 point mutations that do not affect unc-119 rescue, either when present as an array or as a single copy insert.
DNA (Catalog # 114271-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 114271-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMLS253 was a gift from Erik Jorgensen (Addgene plasmid # 114271 ; http://n2t.net/addgene:114271 ; RRID:Addgene_114271) -
For your References section:
SapTrap vectors for introducing point mutations with unc-119+ selection. Schwartz M, Jorgensen E. MicroPubl Biol. 2018 Jul 29;2018:10.17912/DDVH-BG64. doi: 10.17912/DDVH-BG64. 10.17912/DDVH-BG64 PubMed 32550388
Map uploaded by the depositor.
