pGEX-gp78c/2767
(Plasmid #11429)

• Depositing Lab: Allan Weissman
• Publication: Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7.

Backbone

• Vector backbone: pGEX-4T2
• Backbone manufacturer: Amersham/GE
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gp78c
• Alt name: AMFR
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: n/a
• GenBank ID: AF124145
• Entrez Gene: AMFR (a.k.a. GP78, RNF45)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: Amersham 5' pGEX
• 3′ sequencing primer: Amersham 3' pGEX

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A Raz, PMID 96466414

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry