pAB(EXPR-DS-HDAC5_HDAC3)
(Plasmid #114399)

• Purpose: For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression
• Depositing Lab: Vincent Dion
• Publication: Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255.

Backbone

• Vector backbone: pAAV-CMV-GFP
• Backbone size w/o insert (bp): 4974
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PYL-HDAC5_HDAC3 catalytic domain swap
• Species: H. sapiens (human)
• Insert Size (bp): 3962
• Entrez Gene: HDAC3 (a.k.a. HD3, KDAC3, RPD3, RPD3-2)
• Entrez Gene: HDAC5 (a.k.a. HD5, NY-CO-9)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFlag-NLS (internal)

Cloning Information

• Cloning method: Gateway Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry