PurposeMammalian Expression Plasmid of anti-RGS14 (Rat). Derived from hybridoma N133/21.2.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114519||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameanti-RGS14 (Rattus norvegicus) recombinant mouse monoclonal antibody
SpeciesM. musculus (mouse)
- Promoter dual CMV
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
RRID: AB_2750713. Isotype: IgG2a. Additional species: rat, mouse.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Anti-RGS14 [N133/21.2R] was a gift from James Trimmer (Addgene plasmid # 114519 ; http://n2t.net/addgene:114519 ; RRID:Addgene_114519)
For your References section:A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360