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NeuroMab/Trimmer Lab Recombinant mAb and Nanobody Plasmid Collection

The UC Davis/NIH NeuroMab Facility together with the laboratory of NeuroMab Director, Dr. James Trimmer, have produced numerous monoclonal antibodies (NeuroMabs) extensively validated for neuroscience research applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary antibodies. The plasmids encoding these R-mAbs have been deposited with Addgene for distribution to the research community. Protocols for the generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below.

Schematic of antibody plasmid

With these plasmids researchers will be able to easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002.

The schematic to the right shows the separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived VL and VH domain PCR products are fused to a joining fragment comprising a κ light chain constant domain (CL) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion into the p1316 plasmid that contains an upstream CMV promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain across the ER membrane. Downstream of the insert is a heavy chain constant domain (CH) that is either γ1 or γ2a depending on the plasmid, followed by the SV40 polyA tail (SV40 pA).

Recombinant Monoclonal Antibody Plasmid Collection

The following plasmids can be used to produce R-mAbs from transfected mammalian cells.

Addgene ID Plasmid Target Target Species Host Species Isotype
114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a
114474 Anti-Clcn4 [N222/6R] Clcn4 Human Mouse IgG2a
114475 Anti-PARIS/ZNF746 [N196/16R] PARIS/ZNF746 Human Mouse IgG2a
114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a
114477 Anti-TRPML3/Mucolipin-3 [N268/19R] TRPML3/Mucolipin-3 Human Mouse IgG2a
114478 Anti-GABA(A)R, Epsilon [N413/67R] GABA(A)R, Epsilon Human Mouse IgG2a
114479 Anti-Ankyrin-R [N388A/10R] Ankyrin-R Human Mouse IgG2a
114480 Anti-SUR2B [N323B/20R] SUR2B Rat Mouse IgG2a
114481 Anti-QKI-5 [N195A/16R] QKI-5 Human Mouse IgG2a
114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a
114483 Anti-GluA1/GluR1 glutamate receptor [N355/1R] GluA1/GluR1 glutamate receptor Rat Mouse IgG2a
114484 Anti-Stonin-2 [N346/9R] Stonin-2 Human Mouse IgG2a
114485 Anti-NSD3 [N348/82R] NSD3 Human Mouse IgG2a
114486 Anti-Flrt3 [N353/13R] Flrt3 Mouse Mouse IgG2a
114487 Anti-Botch [N116/14R] Botch Rat Mouse IgG2a
114488 Anti-Dopamine D3 receptor [N331/19R] Dopamine D3 receptor Human Mouse IgG2a
114490 Anti-Kirrel3, short and long [N321C/49R] Kirrel3, short and long Rat Mouse IgG2a
114492 Anti-GFP [N86/38.1R] GFP Mouse IgG2a
114493 Anti-NGL-3/LRRC4B [N51/6.2R] NGL-3/LRRC4B Rat Mouse IgG2a
114494 Anti-VGlut3 [N34/34.2R] VGlut3 Rat Mouse IgG2a
114495 Anti-Foxi3 [N359/28R] Foxi3 Mouse Mouse IgG2a
114496 Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R] KCNT2/Slo2.1/Slick K+ channel Mouse Mouse IgG2a
114497 Anti-Cavbeta1 Ca2+ channel [N7/18.1R] Cavbeta1 Ca2+ channel Human Mouse IgG2a
114499 Anti-SVOP [N356/23R] SVOP Rat Mouse IgG2a
114501 Anti-VGlut2 [N29/29R] VGlut2 Rat Mouse IgG2a
114502 Anti-Copper ATPase 1 (Menke's disease protein) [L60/4R] Copper ATPase 1 (Menke's disease protein) Human Mouse IgG2a
114503 Anti-LRRK1 [N341/37R] LRRK1 Human Mouse IgG2a
114505 Anti-Navbeta4 Na+ channel [N168/6.3R] Navbeta4 Na+ channel Rat Mouse IgG2a
114506 Anti-HCN4 [N114/10.1R] HCN4 Human Mouse IgG2a
114507 Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin binding domain, Type I/II) Human Mouse IgG2a
114508 Anti-GluN2B/NR2B glutamate receptor [N59/36.1R] GluN2B/NR2B glutamate receptor Rat Mouse IgG2a
114509 Anti-Pan-FHF-A [N235/22R] Pan-FHF-A Human Mouse IgG2a
114510 Anti-REEP1/2 [N326D/29R] REEP1/2 Mouse Mouse IgG2a
114511 Anti-Kv3.1b K+ channel [N16B/8R] Kv3.1b K+ channel Rat Mouse IgG2a
114512 Anti-HCN1 [N70/28R] HCN1 Rat Mouse IgG2a
114513 Anti-Malin [N85/18.1R] Malin Human Mouse IgG2a
114514 Anti-DNMT3L [N117/9.5R] DNMT3L Human Mouse IgG2a
114515 Anti-GABA(A)R, Delta [N151/3.3R] GABA(A)R, Delta Rat Mouse IgG2a
114516 Anti-Doc2b [N150/21.3R] Doc2b Rat Mouse IgG2a
114517 Anti-SNAT1 [N104/32.1R] SNAT1 Rat Mouse IgG2a
114518 Anti-Neuroligin-3 [N110/29.4R] Neuroligin-3 Rat Mouse IgG2a
114519 Anti-RGS14 [N133/21.2R] RGS14 Rat Mouse IgG2a
114520 Anti-Cav3.1 Ca2+ channel [N178A/9.1R] Cav3.1 Ca2+ channel Mouse Mouse IgG2a
114521 Anti-Pan-QKI [N147/6.1R] Pan-QKI Human Mouse IgG2a
114525 Anti-FGF13/FHF2 [N91/27.1R] FGF13/FHF2 Human Mouse IgG2a
114527 Anti-JIP-2/IB-2 [N135/37.2R] JIP-2/IB-2 Mouse Mouse IgG2a
114529 Anti-Pan-SAPAP [N127/31.1R] Pan-SAPAP Rat Mouse IgG2a
114530 Anti-Neurexin-1-Beta [N170A/1.1R] Neurexin-1-Beta Human Mouse IgG2a
114533 Anti-Laforin [N84/37.1R] Laforin Human Mouse IgG2a
114536 Anti-GFAP R416WT [N206B/9R] GFAP R416WT Human Mouse IgG2a
114538 Anti-FGF12/FHF1 [N94/17R] FGF12/FHF1 Human Mouse IgG2a
114539 Anti-MESD [N88/12.1R] MESD Mouse Mouse IgG2a
114540 Anti-Olig1 [N149/25.1R] Olig1 Mouse Mouse IgG2a
114541 Anti-KCNU1/Slo3 maxi-K+ channel [N2/16R] KCNU1/Slo3 maxi-K+ channel Mouse Mouse IgG2a
114542 Anti-LRP4 (cytoplasmic) [N164/6.1R] LRP4 (cytoplasmic) Mouse Mouse IgG2a
114544 Anti-GABA(B)R2 [N81/37.1R] GABA(B)R2 Human Mouse IgG2a
114547 Anti-Mitofusin-2 [N153/5.1R] Mitofusin-2 Mouse Mouse IgG2a
114548 Anti-Mortalin/GRP75 [N52A/42.1R] Mortalin/GRP75 Mouse Mouse IgG2a
114549 Anti-GABA(A)R, Beta1 [N96/55.3R] GABA(A)R, Beta1 Mouse Mouse IgG2a
114551 Anti-INPP4b [N171/17.4R] INPP4b Human Mouse IgG2a
114552 Anti-Pan-Nav1 Na+ channel [N419/40R] Pan-Nav1 Na+ channel Human Mouse IgG2a
114553 Anti-Pan-Nav1 Na+ channel [N419/78R] Pan-Nav1 Na+ channel Human Mouse IgG2a
114554 Anti-Neuroligin-1 [N97A/31R] Neuroligin-1 Rat Mouse IgG2a
114555 Anti-MMACHC [N230/21R] MMACHC Human Mouse IgG2a
114556 Anti-SUR1 [N289/16R] SUR1 Rat Mouse IgG2a
114557 Anti-TRIP8b (constant) [N212/17R] TRIP8b (constant) Rat Mouse IgG2a
114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a
114559 Anti-Ataxin-1, 11NQ [N76/3R] Ataxin-1, 11NQ Mouse Mouse IgG2a
114560 Anti-S100A5 [N176A/35.1R] S100A5 Rat Mouse IgG2a
114561 Anti-QKI-6 [N182/17.1R] QKI-6 Human Mouse IgG2a
114562 Anti-SynCAM4 [N244/5R] SynCAM4 Mouse Mouse IgG2a
114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a
128612 Anti-Kv2.1 K+ Channel [D3/71R] Kv2.1 K+ Channel Rat Mouse IgG2a
128613 Anti-Kv1.4 K+ channel [K13/31R] Kv1.4 K+ Channel Rat Mouse IgG2a
128614 Anti-Kv1.2 K+ Channel [K14/16R] Kv1.2 K+ Channel Human Mouse IgG2a
128615 Anti-Kvbeta2/KCNAB2 K+ channel [K17/70R] Kvbeta2/KCNAB2 K+ Channel Mouse Mouse IgG2a
128616 Anti-Kv1.1 K+ channel [K20/78R] Kv1.1 K+ Channel Rat Mouse IgG2a
128617 Anti-Pan-Kvbeta K+ channel [K25/73R] Pan-Kvbeta K+ Channel Mouse IgG2a
128618 Anti-PSD-95 [K28/43R] PSD-95 Human Mouse IgG2a
128619 Anti-Kv1.1 K+ channel (External) [K36/15R] Kv1.1 K+ Channel (External) Rat Mouse IgG2a
128620 Anti-Kv2.1 K+ channel (External) [K39/25R] Kv2.1 K+ Channel (External) Human Mouse IgG2a
128621 K51/1RAnti-SH3GL1/Endophilin A2 [K51/1R] SH3GL1/Endophilin A2 Rat Mouse IgG2a
128622 Anti-Kv4.2 K+ Channel (External) [K57/1R] Kv4.2 K+ Channel (External) Human Mouse IgG2a
128623 Anti-Pan-Nav channel [K58/35R] Pan-Nav Channel Mouse IgG2a
128624 Anti-SAP97 [K64/15R] SAP97 Rat Mouse IgG2a
128625 Anti-CASPR/Neurexin IV [K66/38R] CASPR/Neurexin IV Rat Mouse IgG2a
128626 Anti-Nav1.1 Na+ channel [K74/71R] Nav1.1 Na+ channel Rat Mouse IgG2a
128627 Anti-Kv4.3 K+ channel [K75/41R] Kv4.3 K+ Channel Rat Mouse IgG2a
128628 Anti-Kv2.1 K+ Channel [K89/34R] Kv2.1 K+ Channel Rat Mouse IgG2a
128629 Anti-WAVE1/SCAR [K91/36R] WAVE1/SCAR Human Mouse IgG2a
128630 Anti-PICK1 [L20/8R] PICK1 Human Mouse IgG2a
128632 Anti-SynDIG1/Tmem90b [L42/17R] SynDIG1/Tmem90b Mouse Mouse IgG2a
128633 Anti-DLX1 [L43/40R] DLX1 Human Mouse IgG2a
128634 Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) [L6/23R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a
128635 Anti-Ankyrin-G (staining) [N106/43R] Ankyrin-G (staining) Human Mouse IgG2a
128636 Anti-LRRTM4 [N205B/22R] LRRTM4 Human Mouse IgG2a
128637 Anti-Pan-Shank [N23B/49R] Pan-Shank Mouse IgG2a
128638 Anti-VGlut1 [N28/9R] VGlut1 Rat Mouse IgG2a
128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a
128640 Anti-Pan-Ankyrin [N388A/60R] Pan-Ankyrin Mouse IgG2a
128641 Anti-Ataxin-1, 11NQ (Mouse) [N76/8R] Ataxin-1, 11NQ Mouse Mouse IgG2a
140063 Anti-Kv2.1 K+ channel pS603 [L61/14R] Kv2.1 K+ channel pS603 Mouse Mouse IgG2a
140064 Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R] KCNT1/Slo2.2/Slack K+ channel Rt Mouse IgG2a
140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a
140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a
140068 Anti-Kv1.6 K+ channel [K19/36R] Kv1.6 K+ channel Rat Mouse IgG2a
140069 Anti-Kv1.5 K+ channel [K7/45R] Kv1.5 K+ channel Rat Mouse IgG2a
140070 Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] Kvbeta1.1/KCNAB1 K+ channel Mouse Mouse IgG2a
140071 Anti-Snapin/SNAPAP [L8/15R] Snapin/SNAPAP Human Mouse IgG2a
140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a
140073 Anti-Pannexin-2 [N121A/31R] Pannexin-2 Rat Mouse IgG2a
140074 Anti-Kir2.2 K+ channel [N124B/38R] Kir2.2 K+ channel Rat Mouse IgG2a
140075 Anti-Mad3 [N129A/6R] Mad3 Human Mouse IgG2a
140076 Anti-Arl13b [N295B/66R] Arl13b? Mouse Mouse IgG2a
140077 Anti-ZNF423 [N328B/37R] ZNF423 Human Mouse IgG2a
140078 Anti-Shank3 [N69/46R] Shank3 Rat Mouse IgG2a
140079 Anti-GIT2 [N83/48R] GIT2 Human Mouse IgG2a
140081 Anti-Neuroligin-4* [N98/7R] Neuroligin-4* Mouse Mouse IgG2a
149451 Anti-GluK5/Grik5/KA2 kainate receptor [N279B/27R] GluK5/Grik5/KA2 kainate receptor Rat Mouse IgG2a
149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a
149453 Anti-Kv4.2 K+ channel [L28/4R] Kv4.2 K+ channel Rat Mouse IgG2a
149454 Anti-BK beta4 K+ channel [L18A/3R] BK beta4 K+ channel Mouse Mouse IgG2a
149455 Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor Rat Mouse IgG2a
149456 Anti-GABA(A)R, Alpha5 [N415/24R] GABA(A)R, Alpha5 Human Mouse IgG2a
149458 Anti-Cavbeta2 Ca2+ channel [N8B/1R] Cavbeta2 Ca2+ channel Rat Mouse IgG2a
164154 Anti-TRIP8b [N212/7R] TRIP8b Rat Mouse IgG2a
164155 Anti-GABA(A)R, alpha6 [N229A/32R] GABA(A)R, alpha6 Mouse Mouse IgG2a
164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a
164157 Anti-Iduna/RNF146 [N201/35R] Iduna/RNF146 Mouse Mouse IgG2a
164158 Anti-DNAH7 [N250/21.4R] DNAH7 Human Mouse IgG2a

Browse Addgene’s full antibody and nanobody collection here.

Nanobody Plasmid Collection

The following plasmids can be used to produce nanobodies from transfected bacterial cells.

Addgene ID Plasmid Hybridoma Target Target Species Host Species
134715 HS63 pComb3xss anti-Homer1 nanobody HS63 Homer1 Mouse Llama
134716 HS69 pComb3xss anti-Homer1 nanobody HS69 Homer1 Mouse Llama
134717 HS89 pComb3xss anti-Homer1 nanobody HS89 Homer1 Mouse Llama
134718 HC25 pComb3xss anti-Homer1 nanobody HC25 Homer1 Mouse Llama
134719 HC89 pComb3xss anti-Homer1 nanobody HC89 Homer1 Mouse Llama
134720 GC1 pComb3xss anti-Gephyrin nanobody GC1 Gephyrin Mouse Llama
134721 GC83 pComb3xss anti-Gephyrin nanobody GC83 Gephyrin Mouse Llama
135219 HS22 pEYFPN1 anti-Homer1 nanobody HS22 Homer1 Mouse Llama
135220 HC20 pEYFPN1 anti-Homer1 nanobody HC20 Homer1 Mouse Llama
135221 HS38 pEYFPN1 anti-Homer1 nanobody HS38 Homer1 Mouse Llama
135222 HC12 pEYFPN1 anti-Homer1 nanobody HC12 Homer1 Mouse Llama
135223 HC87 pEYFPN1 anti-Homer1 nanobody HC87 Homer1 Mouse Llama
135224 GC52 pEGFPN1 anti-Gephyrin nanobody GC52 Gephyrin Mouse Llama
135225 AC50 pEGFPN1 anti-AMIGO-1 nanobody AC50 AMIGO-1 Mouse Llama
135226 AC27 pEGFPN1 anti-AMIGO-1 nanobody AC27 AMIGO-1 Mouse Llama
135227 IC65 pEGFPN1 anti-IRSp53 nanobody IC65 IRSp53 Mouse Llama
135228 SS80 pEGFPN1 anti-SAPAP2 nanobody SS80 SAPAP2 Mouse Llama
145818 SS1 pComb3xss anti-SAPAP2 nanobody SS1 SAPAP2 Rat Llama
145819 SC27 pComb3xss anti-SAPAP2 nanobody SC27 SAPAP2 Rat Llama
145820 SS2 pComb3xss anti-SAPAP2 nanobody SS2 SAPAP2 Rat Llama
145821 SS3 pComb3xss anti-SAPAP2 nanobody SS3 SAPAP2 Rat Llama
145822 SS32 pComb3xss anti-SAPAP2 nanobody SS32 SAPAP2 Rat Llama
145823 SS41 pComb3xss anti-SAPAP2 nanobody SS41 SAPAP2 Rat Llama
145824 SS44 pComb3xss anti-SAPAP2 nanobody SS44 SAPAP2 Rat Llama
145825 SS88 pComb3xss anti-SAPAP2 nanobody SS88 SAPAP2 Rat Llama
145826 GC92 pComb3xss anti-Gephyrin nanobody GC92 Gephyrin Human Llama
145827 GS8 pComb3xss anti-Gephyrin nanobody GS8 Gephyrin Human Llama
145828 GS41 pComb3xss anti-Gephyrin nanobody GS41 Gephyrin Human Llama
145829 C1-82 pComb3xss anti-cFos nanobody C1-82 cFos Human Llama
145830 C1-84 pComb3xss anti-cFos nanobody C1-84 cFos Human Llama
145831 C2-58 pComb3xss anti-cFos nanobody C2-58 cFos Human Llama
145832 S1-71 pComb3xss anti-cFos nanobody S1-71 cFos Human Llama
145833 C2-66 pComb3xss anti-cFos nanobody C2-66 cFos Human Llama
145834 S2-77 pComb3xss anti-cFos nanobody S2-77 cFos Human Llama
145835 C1-62 pComb3xss anti-cFos nanobody C1-62 cFos Human Llama
145836 S1-54 pComb3xss anti-cFos nanobody S1-54 cFos Human Llama
145837 C2-77 pComb3xss anti-cFos nanobody C2-77 cFos Human Llama
145838 C2-82 pComb3xss anti-cFos nanobody C2-82 cFos Human Llama

Browse Addgene’s full antibody and nanobody collection here.


The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for working with R-mAbs expressed from the plasmids in this collection.

UC Davis/NIH NeuroMab Protocols


Andrews NP, et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife. 8:e43322. PMID: 30667360

Crosnier C, et al. 2010. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biology. 8:76. PMID: 20525357

Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 30:e9. PMID: 11788735

Trimmer J, et al. 2020. Recombinant Antibodies in Basic Neuroscience Research. Curr Prot Neurosci. 94.1. PMID: 33151027

Dong JX, et al. 2019. A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons. eLife. 8:e48750. PMID: 31566565

Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research. Curr Prot Neurosci. 94.1. PMID: 33185319

UC Davis/NIH NeuroMab Publications