Anti-GABA(B)R2 [N81/37.1R]
(Plasmid #114544)

• Purpose: Mammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.
• Depositing Lab: James Trimmer
• Publication: Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322.

Backbone

• Vector backbone: P1316-IgG2a
  (Search Vector Database)
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 5925
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: anti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody
• Species: M. musculus (mouse)
• GenBank ID: O75899
• Entrez Gene: GABBR2 (a.k.a. DEE59, EIEE59, GABABR2, GPR51, GPRC3B, HG20, HRIHFB2099, NDPLHS)
• Promoter: dual CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RRID: AB_2750815. Isotype: IgG2a. Additional species: human, mouse, rat.

A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.