N81/37.1R
(Plasmid
#114544)
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PurposeMammalian Expression of anti-GABA(B)R2 (Human) recombinant mouse monoclonal antibody
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 114544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneP1316-IgG2a
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameanti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody
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SpeciesM. musculus (mouse)
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GenBank IDO75899
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Entrez GeneGABBR2 (a.k.a. EIEE59, GABABR2, GPR51, GPRC3B, HG20, HRIHFB2099, NDPLHS)
- Promoter dual CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Depositor Comments
RRID: AB_2750815. Isotype: IgG2a. Additional species: human, mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N81/37.1R was a gift from James Trimmer (Addgene plasmid # 114544 ; http://n2t.net/addgene:114544 ; RRID:Addgene_114544) -
For your References section:
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360