Anti-GFP [N86/8R]
(Plasmid #177572)

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Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.
Depositing Lab

James Trimmer
Publication

NeuroMab/Trimmer Lab Recombinant mAb Plasmids (unpublished) ( How to cite )
Sequence Information
- Sequences (3) — Accept Affinity Reagent Sequence Policy

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	177572	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
P1316-IgG2a
(Search Vector Database)
Backbone manufacturer
Gavin Wright, Sanger; Yves Durocher, NRC
Backbone size w/o insert (bp) 5925
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
Species
M. musculus (mouse)
Promoter Dual CMV

Cloning Information

Cloning method Gibson Cloning

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a recombinant antibody derived from RRID: AB_2313651. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

Anti-GFP [N86/8R] was a gift from James Trimmer (Addgene plasmid # 177572 ; http://n2t.net/addgene:177572 ; RRID:Addgene_177572)

Anti-GFP [N86/8R] (Plasmid #177572)