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N153/5.1R
(Plasmid #114547)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114547 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    P1316-IgG2a
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 5925
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-Mitofusin-2 (Mus musculus) recombinant mouse monoclonal antibody
  • Species
    M. musculus (mouse)
  • GenBank ID
    Q80U63
  • Entrez Gene
    Mfn2 (a.k.a. D630023P19Rik, Fzo)
  • Promoter dual CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

RRID: AB_2750725. Isotype: IgG2a. Additional species: mouse, rat.

A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N153/5.1R was a gift from James Trimmer (Addgene plasmid # 114547 ; http://n2t.net/addgene:114547 ; RRID:Addgene_114547)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360