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HC87 pEYFPN1 anti-Homer1 nanobody
(Plasmid #135223)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135223 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-Homer1 (Mouse) recombinant llama nanobody HC87
  • Species
    L. glama (llama)
  • Entrez Gene
    Homer1 (a.k.a. PSD-Z, PSD-Zip45, SYN47, Ves, Ves-1, homer-1, vesl-1)
  • Tag / Fusion Protein
    • 6xHis-HA-EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HC87 pEYFPN1 anti-Homer1 nanobody was a gift from James Trimmer (Addgene plasmid # 135223 ; http://n2t.net/addgene:135223 ; RRID:Addgene_135223)
  • For your References section:

    A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons. Dong JX, Lee Y, Kirmiz M, Palacio S, Dumitras C, Moreno CM, Sando R, Santana LF, Sudhof TC, Gong B, Murray KD, Trimmer JS. Elife. 2019 Sep 30;8. pii: 48750. doi: 10.7554/eLife.48750. 10.7554/eLife.48750 PubMed 31566565