Anti-FGF13/FHF2, B isoform [N225A/10R]
(Plasmid #188208)

• Purpose: Mammalian Expression Plasmid of anti-FGF13/FHF2, B isoform (Human). Derived from hybridoma N225A/10
• Depositing Lab: James Trimmer
• Publication: NeuroMab/Trimmer Lab Recombinant mAb Plasmids (unpublished)

Backbone

• Vector backbone: P1316-IgG2a
  (Search Vector Database)
• Backbone manufacturer: Gavin Wright, Sanger; Yves Durocher, NRCC
• Backbone size w/o insert (bp): 5925
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: anti-FGF13/FHF2, B isoform (Homo sapiens) recombinant mouse monoclonal antibody
• Species: M. musculus (mouse)
• Entrez Gene: FGF13 (a.k.a. DEE90, FGF-13, FGF2, FHF-2, FHF2, LINC00889)
• Promoter: Dual CMV

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a recombinant antibody derived from RRID: AB_2916243. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.