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(Plasmid #11466)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 11466 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    see PMID 1852137
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    aa 52-777
  • GenBank ID
  • Entrez Gene
    Nedd4 (a.k.a. AA959633, AL023035, AU019897, E430025J12Rik, EG639396, Gm7265, KIAA0093, Nedd4-1, Nedd4a)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer Amersham 5' pGEX
  • 3′ sequencing primer Amersham 3' pGEX
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-Nedd-4/2522 was a gift from Allan Weissman (Addgene plasmid # 11466 ; ; RRID:Addgene_11466)
  • For your References section:

    Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. Hatakeyama S, Jensen JP, Weissman AM. J Biol Chem. 1997 Jun 13. 272(24):15085-92. 10.1074/jbc.272.24.15085 PubMed 9182527