pGEX-Nedd4-N / 2524 JM16
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13312||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Mutationaa1-52 and aa422-777 deleted
Entrez GeneNedd4 (a.k.a. AA959633, AL023035, AU019897, E430025J12Rik, EG639396, Gm7265, KIAA0093, Nedd4-1, Nedd4a)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pGEX 5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
C-terminal truncation generated by digestion and re-ligation into vector. Therefore, the protein will contain vector-derived amino acids at the C-terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-Nedd4-N / 2524 JM16 was a gift from Allan Weissman (Addgene plasmid # 13312 ; http://n2t.net/addgene:13312 ; RRID:Addgene_13312)
For your References section:Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. Hatakeyama S, Jensen JP, Weissman AM. J Biol Chem. 1997 Jun 13. 272(24):15085-92. 10.1074/jbc.272.24.15085 PubMed 9182527