pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
(Plasmid
#115207)
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PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression construct
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneSUMO bacterial expression vector
- Backbone size w/o insert (bp) 6087
- Total vector size (bp) 9759
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBzoCas13
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Alt nameCas13a from Bergeyella zoohelcum
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SpeciesBergeyella zoohelcum
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Insert Size (bp)3672
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Tag
/ Fusion Protein
- SUMO (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI was a gift from Feng Zhang (Addgene plasmid # 115207 ; http://n2t.net/addgene:115207 ; RRID:Addgene_115207) -
For your References section:
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. 10.1126/science.aaq0179 PubMed 29449508
