pLV-U-TVA950
(Plasmid
#115236)
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PurposeLentiviral transfer plasmid for expression of TVA950
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 115236-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 115236-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCSC-SP-PW-GFP
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Backbone manufacturerInder Verma Lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTVA950
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SpeciesSynthetic
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Insert Size (bp)474
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 115236-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 115236-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-U-TVA950 was a gift from Ian Wickersham (Addgene plasmid # 115236 ; http://n2t.net/addgene:115236 ; RRID:Addgene_115236) -
For your References section:
"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification. Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, Pruner MT, Dam Ferdinez ML, Wickersham IR. Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 10.1073/pnas.2023481120. Epub 2023 Feb 6. 10.1073/pnas.2023481120 PubMed 37053554
