PurposeFor mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115252||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
Entrez GeneNODAL (a.k.a. HTX5)
- Promoter CMV
/ Fusion Protein
- MYC-DYK (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Both N-glycosylation sites in the NODAL pro-domain are mutated . Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NODAL-MYC-DYK-N72A-N199A was a gift from Lynne Postovit (Addgene plasmid # 115252 ; http://n2t.net/addgene:115252 ; RRID:Addgene_115252)
For your References section:Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct. Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L. (2018) bioRxiv 276170 10.1101/276170