Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct
Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L
(2018) bioRxiv 276170 Article
Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L
(2018) bioRxiv 276170 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115245 | NODALvar-MYC-DYK | For mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tag |
| 115246 | NODALvar-MYC-DYK-N328A | For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag |
| 115248 | NODAL-matMYC | For mammalian expression of the human NODAL open reading frame with an internal MYC tag |
| 115249 | NODALvar-matMYC | For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag |
| 115250 | NODAL-T7TS | For in vitro transcription of human NODAL open reading frame RNA |
| 115251 | NODALvar-T7TS | For in vitro transcription of a human NODAL splice variant open reading frame RNA |
| 115252 | NODAL-MYC-DYK-N72A-N199A | For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag |
| 115253 | NODAL-MYC-DYK-C312A | For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag |
| 115254 | NODALvar-MYC-DYK-C302S | For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag |
| 115255 | NODALvar-MYC-DYK-C302A | For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag |
| 115256 | NODAL-matMYC-C312S | For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal MYC tag |
| 115257 | NODAL-matMYC-ex2trunc | For mammalian expression of a C-terminal truncated human NODAL open reading frame with an internal MYC tag |
| 115258 | NODAL-matDYK | For mammalian expression of the human NODAL open reading frame with an internal DYK tag |
| 115259 | NODAL-matDYK-C312S | For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal DYK tag |
| 115260 | NODALvar-matDYK | For mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tag |
| 115262 | NODALvar-CAG-AAVS1 | For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells |
| 115263 | NODAL-T7TS-C312S | For in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNA |
| 115264 | NODAL-T7TS-N72A-N199A | For in vitro transcription of human NODAL open reading frame (with two mutated N-glycosylation sites) RNA |
| 115265 | pET28a-matNODALvar-HIS | For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant |
| 115638 | AAVS1-TRE3G-NODAL | For targeted integration and inducible expression of human NODAL using doxycycline |
| 115639 | AAVS1-TRE3G-NODAL-matMYC | For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline |
| 115640 | AAVS1-TRE3G-NODALvar | For targeted integration and inducible expression of a human NODAL splice variant using doxycycline |
| 115641 | AAVS1-TRE3G-NODALvar-matMYC | For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline |