PurposeFor in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115263||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerPaul Krieg, Addgene plasmid # 17091
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Entrez GeneNODAL (a.k.a. HTX5)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
MCS lacks PstI, SalI and AccI sites.
Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NODAL-T7TS-C312S was a gift from Lynne Postovit (Addgene plasmid # 115263 ; http://n2t.net/addgene:115263 ; RRID:Addgene_115263)
For your References section:Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct. Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L. (2018) bioRxiv 276170 10.1101/276170