PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variant
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115265||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameNODAL variant mature peptide
SpeciesH. sapiens (human)
Entrez GeneNODAL (a.k.a. HTX5)
/ Fusion Protein
- 6His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-matNODALvar-HIS was a gift from Lynne Postovit (Addgene plasmid # 115265 ; http://n2t.net/addgene:115265 ; RRID:Addgene_115265)
For your References section:Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct. Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L. (2018) bioRxiv 276170 10.1101/276170