PurposeFor targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115641||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Growth instructionsCan be grown at 37°C in TOP10 E. coli.
Copy numberHigh Copy
Gene/Insert nameNODAL splice variant
SpeciesH. sapiens (human)
Entrez GeneNODAL (a.k.a. HTX5)
- Promoter TRE3G
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer TRE3G (GATCGCCTGGAGCAATTCCAC)
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Use with TALENs #52341 and #52342. Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAVS1-TRE3G-NODALvar-matMYC was a gift from Lynne Postovit (Addgene plasmid # 115641 ; http://n2t.net/addgene:115641 ; RRID:Addgene_115641)
For your References section:Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct. Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L. (2018) bioRxiv 276170 10.1101/276170