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pLTR.gp140/EGFP.Revdelta38/DsRed
(Plasmid #115775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115775 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 115775-D50 50 μg of DNA in Tris buffer $495
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DNA 115775-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
  • Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated prior to the transmembrane domain at amino acid 1363); Revdelta38
  • Promoter HIV-1 (LTR)
  • Tag / Fusion Protein
    • EGFP; dsReD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see Khoury et al. Retrovirology (2018) 15:36
https://doi.org/10.1186/s12977-018-0421-6

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLTR.gp140/EGFP.Revdelta38/DsRed was a gift from Damian Purcell (Addgene plasmid # 115775 ; http://n2t.net/addgene:115775 ; RRID:Addgene_115775)

  • For your References section:

    High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing. Khoury G, Purcell DFJ. J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753. 10.3791/58753 PubMed 30735158
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