NA20 LN16
(Plasmid #101870)

• Purpose: Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus
• Depositing Lab: Paul Clapham
• Publication: Peters et al J Virol. 2004 Jul;78(13):6915-26.

Backbone

• Vector backbone: pSVIIIenv
• Backbone manufacturer: NIH Aids Reagent Program
• Total vector size (bp): 7989

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Env
• Alt name: HIV envelope glycoprotein
• Insert Size (bp): 2400
• GenBank ID: HM014325.1
• Entrez Gene: env (a.k.a. HIV1gp8)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: Env A
• 3′ sequencing primer: Env N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Paul Peters, UMass Medical School, Worcester MA
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry