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PurposeMeasles virus hemagglutinin expressing plasmid
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Depositing Lab
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Depositing OrganizationFDA, Center for Biologics Evaluation and Research (CBER)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 84817-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 84817-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMeasles virus H glycoprotein
- Promoter CMV IE
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CTCCGCCCCATTGACGCA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Andrea Maisner, Philipps University of Marburg, 35043 Marburg Germany
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 84817-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 84817-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG-Hc∆18 was a gift from Jakob Reiser (Addgene plasmid # 84817 ; http://n2t.net/addgene:84817 ; RRID:Addgene_84817) -
For your References section:
A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins. Marino MP, Panigaj M, Ou W, Manirarora J, Wei CH, Reiser J. Gene Ther. 2015 Mar;22(3):280-5. doi: 10.1038/gt.2014.125. Epub 2015 Jan 22. 10.1038/gt.2014.125 PubMed 25608718
Map uploaded by the depositor.
