pCMV-hAR-R617M,K618M,K632M,K633M
(Plasmid #115778)

• Purpose: Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells
• Depositing Lab: Elizabeth Wilson
• Publication: Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 7300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Androgen Receptor
• Alt name: AR
• Species: H. sapiens (human)
• Mutation: R617M, K618M ,K632M, K633M, insertion of Q81 and Q82
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert encodes 25 glutamines in the polymorphic region of the androgen receptor. Mutations are based on the numbering from the associated publication.