LSL K-ras G12D
(Plasmid #11585)

• Depositing Lab: Tyler Jacks
• Publication: Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

Backbone

• Vector backbone: n/a
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid grows at 37oC, but plasmid yield and stability is improved when grown at 30oC.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: K-ras G12D
• Alt name: K-ras
• Species: M. musculus (mouse)
• Mutation: G12D. Oncogenic Form.
• Entrez Gene: Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid containing K-ras G12D with a LoxP-STOP-LoxP cassette included upstream of the K-ras sequence such that K-ras G12D will only be expressed after cre-mediated recombination.

The positive selection for ES cells is puromycin, and negative selection is carried out by addition of ganciclovir.

The plasmid sequence submitted by the author is an approximation. For example, the Bluescript backbone sequence was chosen randomly (e.g. the Sal I site in the backbone is definitely not present in the construct).