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pK-SAN1-Flag
(Plasmid #117163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK7
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 4719
  • Total vector size (bp) 7455
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAN1
  • Alt name
    CCPG; fam120b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2736
  • GenBank ID
    NM_001286379
  • Entrez Gene
    FAM120B (a.k.a. CCPG, KIAA1838, PGCC1, SAN1, dJ894D12.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13fwd
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NBRC, Japan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SAN1 contains a difference compared to the reference sequence NM_001286379 that results in a D370N difference in the protein product.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK-SAN1-Flag was a gift from Ian Macara (Addgene plasmid # 117163 ; http://n2t.net/addgene:117163 ; RRID:Addgene_117163)
  • For your References section:

    A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Andrews AM, McCartney HJ, Errington TM, D'Andrea AD, Macara IG. Nat Commun. 2018 Jul 3;9(1):2592. doi: 10.1038/s41467-018-05008-8. 10.1038/s41467-018-05008-8 [pii] PubMed 29968717