pASK-mSAN1-Streptag
(Plasmid #117165)

• Purpose: Expresses Strep-tagged murine SAN1 nuclease in bacteria
• Depositing Lab: Ian Macara
• Publication: Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 10.1038/s41467-018-05008-8.

Backbone

• Vector backbone: pASK-IBA3Plus
• Backbone manufacturer: IBA Lifesciences
• Backbone size w/o insert (bp): 3172
• Total vector size (bp): 5527
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow at 18oC after induction with tetracycline
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSAN1
• Alt name: CCPG; fam120b
• Species: M. musculus (mouse)
• Insert Size (bp): 2355
• GenBank ID: NM_001357822.1
• Entrez Gene: Fam120b (a.k.a. 4932442K08Rik, AW049283, AW413338, CCPG, Pgcc1, mKIAA1838)
• Promoter: tetR/tetA
• Tag / Fusion Protein:
  • Streptag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bsa1 (not destroyed)
• 3′ cloning site: Bsa1 (not destroyed)
• 5′ sequencing primer: custom

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MGC cDNA library

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MGC cDNA was a partial clone, repaired by the authors to generate full length mSAN1. mSAN1 contains a difference compared to NM_001357822.1 that results in a V549I protein-coding change.