pUC19_18GZG
(Plasmid
#117228)
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Purposeshble flanked by an endogenous GAPDH promoter and terminator system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 2686
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Vector typeThraustochytrid expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshble
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Alt nameBleoR
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SpeciesStreptoalloteichus hindustanus
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Insert Size (bp)375
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GenBank IDX52869.1
- Promoter GAPDH
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SphI, SalI (not destroyed)
- 5′ sequencing primer M13
- 3′ sequencing primer M13 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cloning method is Infusion cloning (Clontech) and restriction digest
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19_18GZG was a gift from Jackie Collier (Addgene plasmid # 117228 ; http://n2t.net/addgene:117228 ; RRID:Addgene_117228) -
For your References section:
Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Faktorova D, Nisbet RER, Fernandez Robledo JA, Casacuberta E, Sudek L, Allen AE, Ares M Jr, Areste C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget FY, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, Garcia PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibanez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano JC, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Novak Vanclova AMG, Nowacki M, Nunez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Jason Smith G, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnsek J, Valach M, Verge V, von Dassow P, von der Haar T, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, Lukes J. Nat Methods. 2020 May;17(5):481-494. doi: 10.1038/s41592-020-0796-x. Epub 2020 Apr 6. 10.1038/s41592-020-0796-x PubMed 32251396
Map uploaded by the depositor.
