pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
(Plasmid
#139961)
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PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanica
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAMBIA-pBR322
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
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SpeciesMultiple
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG was a gift from Raffaela Abbriano (Addgene plasmid # 139961 ; http://n2t.net/addgene:139961 ; RRID:Addgene_139961) -
For your References section:
Overexpression of Key Sterol Pathway Enzymes in Two Model Marine Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum. Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, Ralph PJ. Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 10.3390/ph13120481. 10.3390/ph13120481 PubMed 33371196
