pXS-mPlum
(Plasmid #117388)

• Purpose: pGEN-mcs with a modular mPlum expression scaffold
• Depositing Lab: Karen Guillemin
• Publication: Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-18. doi: 10.1128/mBio.01877-18.

Backbone

• Vector backbone: pGEN-mcs (ori15A)
• Modifications to backbone: Expression scaffold contains modular promoter, 5' UTR, and 3' UTR sequences
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mPlum
• Species: Synthetic
• Insert Size (bp): 681
• Promoter: Ptac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ggctggataaagggaactca
• 3′ sequencing primer: gcgggctagctcattatttg

Resource Information

• Supplemental Documents:
  • pXS-mPlum.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry