4xHRE_MLP-sfGFP-CMV_dsRed Exp
(Plasmid
#117403)
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PurposesfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoter
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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 117403-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 117403-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuperFolder GFP
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Alt namesfGFP
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Insert Size (bp)714
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe sfGFP gene was PCRed from a plasmid received from Dr. Pamela Silver's laboratory.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 117403-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 117403-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xHRE_MLP-sfGFP-CMV_dsRed Exp was a gift from Yvonne Chen (Addgene plasmid # 117403 ; http://n2t.net/addgene:117403 ; RRID:Addgene_117403) -
For your References section:
Quantitative Analyses of Core Promoters Enable Precise Engineering of Regulated Gene Expression in Mammalian Cells. Ede C, Chen X, Lin MY, Chen YY. ACS Synth Biol. 2016 May 20;5(5):395-404. doi: 10.1021/acssynbio.5b00266. Epub 2016 Mar 1. 10.1021/acssynbio.5b00266 PubMed 26883397
