PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117528||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameSp-eCas9 1.1 (with stop codon)
MutationK848A, K1003A, R1060A
- Cloning method Restriction Enzyme
- 5′ cloning site BpiI (destroyed during cloning)
- 3′ cloning site BpiI (destroyed during cloning)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEPOR0SP0018 was a gift from Nicola Patron (Addgene plasmid # 117528 ; http://n2t.net/addgene:117528 ; RRID:Addgene_117528)
For your References section:Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. Raitskin O, Schudoma C, West A, Patron NJ. PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. 10.1371/journal.pone.0211598 PubMed 30811422