Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
Raitskin O, Schudoma C, West A, Patron NJ
PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. PubMed Article
Raitskin O, Schudoma C, West A, Patron NJ
PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117520 | pICSL90005 | Level0 Golden Gate part: CDS, SpCas9-h (no stop codon) |
| 117521 | pEPOR0SP0013 | Level0 Golden Gate part: CDS, SpCas9-p |
| 117522 | pEPOR0SP0009 | Level0 Golden Gate part: CDS, SpCas9-p (no stop codon) |
| 117523 | pEPOR0SP0001 | Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon) |
| 117524 | pEPOR0SP0014 | Level0 Golden Gate part: CDS, SpCas9-KA |
| 117525 | pEPOR0SP0015 | Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon) |
| 117526 | pEPOR0SP0016 | Level0 Golden Gate part: CDS, Sp-eCas9 1.0 |
| 117527 | pEPOR0SP0017 | Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon) |
| 117528 | pEPOR0SP0018 | Level0 Golden Gate part: CDS, Sp-eCas9 1.1 |
| 117529 | pEPOR0SP0019 | Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon) |
| 117530 | pEPOR0SP0011 | Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon) |
| 117531 | pEPOR0SP0020 | Level0 Golden Gate part: CDS, SaCas9 |
| 117532 | pEPOR0SP0021 | Level0 Golden Gate part: CDS, SaCas9 (no stop codon) |
| 117533 | pEPOR0SP0012 | Level0 Golden Gate part: CDS, eSaCas9 (no stop codon) |
| 117534 | pEPOR0SP0007 | Level0 Golden Gate part: CDS, LbCas12a |
| 117535 | pEPOR0SP0008 | Level0 Golden Gate part: CDS, AsCas12a |
| 117536 | pICSL50005 | Level0 Golden Gate part: c-terminal tag, YFP |
| 117537 | pEPOR1CB0022 | Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana |
| 117538 | pEPOR1CB0023 | Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana |
| 117539 | pEPOR1CB0058 | Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana |
| 117540 | pEPOR1CB0028 | Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana |
| 117541 | pEPOR1CB0029 | Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana |
| 117542 | pICSL11023 | Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants |
| 117543 | pEPOR1CB0002 | Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants |
| 117544 | pEPOR1CB0008 | Level 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plants |
| 117545 | pEPOR1CB0009 | Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants |
| 117546 | pEPOR1CB0010 | Level 1 Golden Gate Cassette: eCas9 1.0 expression cassette for dicotyledonous plants |
| 117547 | pEPOR1CB0011 | Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants |
| 117548 | pEPOR1CB0112 | Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants |
| 117549 | pEPOR1CB0013 | Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants |
| 117550 | pEPOR1CB0014 | Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants |
| 117551 | pEPOR1CB0015 | Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants |
| 117552 | pEPOR1CB0113 | Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants |
| 117553 | pEPOR1CB0114 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana |
| 117554 | pEPOR1CB0018 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer |
| 117555 | pEPOR1CB0019 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer |
| 117556 | pEPOR1CB0020 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer |
| 117557 | pEPOR1CB0021 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer |
| 117558 | pEPOR1CB0027 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtADH gene gene in A. thaliana with 1 mismatch at PAM |
| 117559 | pEPOR1CB0071 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting RPS4A family protein {AT5G58420 } in A. thaliana |
| 117560 | pEPOR1CB0075 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Rpn2/Psmd1 subunit {AT2G32730} in A. thaliana |
| 117561 | pEPOR1CB0081 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtMPK19 {AT3G14720} in A. thaliana |
| 117562 | pEPOR1CB0083 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Methyltransferase {AT5G10620} in A. thaliana |
| 117563 | pEPOR1CB0084 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCPK34 {AT5G19360} in A. thaliana |
| 117564 | pEPOR1CB0087 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtUUAT1 {AT5G04160} in A. thaliana |
| 117565 | pEPOR1CB0089 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting {AT1G68250} in A. thaliana |
| 117566 | pEPOR1CB0092 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCas6 {AT5G04770} in A. thaliana |
| 117567 | pEPOR1CB0103 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCas6 {AT5G04770} in A. thaliana |
| 117568 | pEPOR1CB0093 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana |
| 117569 | pEPOR1CB0104 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana |
| 117570 | pEPOR1CB0098 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thaliana |
| 117571 | pEPOR1CB0109 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thaliana |
| 117572 | pEPOR1CB0099 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana |
| 117573 | pEPOR1CB0110 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana |
| 117574 | pEPOR1CB0100 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting ENTH/ANT {AT1G14910} in A. thaliana |
| 117575 | pEPOR1CB0111 | Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting ENTH/ANT {AT2G01600} in A. thaliana |
| 117576 | pEPOR2KN0001 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117577 | pEPOR2KN0002 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas-p(pEPOR1CB0002) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117578 | pEPOR2KN0003 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022) |
| 117579 | pEPOR2KN0004 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023) |
| 117580 | pEPOR2KN0008 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018) |
| 117581 | pEPOR2KN0009 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019) |
| 117582 | pEPOR2KN0010 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020) |
| 117583 | pEPOR2KN0011 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021) |
| 117584 | pEPOR2KN0012 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117585 | pEPOR2KN0013 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018) |
| 117586 | pEPOR2KN0014 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019) |
| 117587 | pEPOR2KN0015 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020) |
| 117588 | pEPOR2KN0016 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021) |
| 117589 | pEPOR2KN0017 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117590 | pEPOR2KN0018 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018) |
| 117591 | pEPOR2KN0019 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019) |
| 117592 | pEPOR2KN0020 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020) |
| 117593 | pEPOR2KN0021 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021) |
| 117594 | pEPOR2KN0022 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117595 | pEPOR2KN0023 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018) |
| 117596 | pEPOR2KN0024 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019) |
| 117597 | pEPOR2KN0025 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020) |
| 117598 | pEPOR2KN0026 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021) |
| 117599 | pEPOR2KN0027 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS(pEPOR1CB0114) |
| 117600 | pEPOR2KN0028 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018) |
| 117601 | pEPOR2KN0029 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019) |
| 117602 | pEPOR2KN0030 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020) |
| 117603 | pEPOR2KN0031 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021) |
| 117604 | pEPOR2KN0032 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0027) |
| 117605 | pEPOR2KN0038 | Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029) |
| 117606 | pEPOR2KN0039 | Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028) |
| 117607 | pEPOR2KN0040 | Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058) |
| 117608 | pEPOR2KN0041 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071) |
| 117609 | pEPOR2KN0042 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071) |
| 117610 | pEPOR2KN0043 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071) |
| 117611 | pEPOR2KN0048 | Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075) |
| 117612 | pEPOR2KN0045 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075) |
| 117613 | pEPOR2KN0046 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075) |
| 117614 | pEPOR2KN0047 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075) |
| 117615 | pEPOR2KN0044 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071) |
| 117616 | pEPOR2KN0049 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081) |
| 117617 | pEPOR2KN0050 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081) |
| 117618 | pEPOR2KN0051 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081) |
| 117619 | pEPOR2KN0052 | Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081) |
| 117620 | pEPOR2KN0053 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083) |
| 117621 | pEPOR2KN0054 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083) |
| 117622 | pEPOR2KN0055 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083) |
| 117623 | pEPOR2KN0056 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083) |
| 117624 | pEPOR2KN0057 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084) |
| 117625 | pEPOR2KN0058 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084) |
| 117626 | pEPOR2KN0059 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084) |
| 117627 | pEPOR2KN0060 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084) |
| 117628 | pEPOR2KN0061 | Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087) |
| 117629 | pEPOR2KN0062 | Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087) |
| 117630 | pEPOR2KN0063 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_pEPOR1CB0089(pEPOR1CB0089) |
| 117631 | pEPOR2KN0064 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_pEPOR1CB0089(pEPOR1CB0089) |
| 117632 | pEPOR2KN0065 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_pEPOR1CB0089(pEPOR1CB0089) |
| 117633 | pEPOR2KN0066 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_pEPOR1CB0089(pEPOR1CB0089) |
| 117634 | pEPOR2KN0067 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103) |
| 117635 | pEPOR2KN0068 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103) |
| 117636 | pEPOR2KN0069 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103) |
| 117637 | pEPOR2KN0070 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103) |
| 117638 | pEPOR2KN0071 | Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104) |
| 117639 | pEPOR2KN0072 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104) |
| 117640 | pEPOR2KN0073 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104) |
| 117641 | pEPOR2KN0074 | Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104) |
| 117642 | pEPOR2KN0075 | Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Flavin-binding monooxygenase family protein {AT1G62600}(pEPOR1CB0098)and sgRNA_Flavin-binding monooxygenase family protein {AT1G62590}(pEPOR1CB0109) |
| 117643 | pEPOR2KN0076 | Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109) |
| 117644 | pEPOR2KN0077 | Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110) |
| 117645 | pEPOR2KN0078 | Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein. |
| 117646 | pEPOR2KN0079 | Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111) |
| 117647 | pEPOR2KN0080 | Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111) |