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Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
Raitskin O, Schudoma C, West A, Patron NJ
PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117520pICSL90005 Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)
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117521pEPOR0SP0013Level0 Golden Gate part: CDS, SpCas9-p
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117522pEPOR0SP0009Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)
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117523pEPOR0SP0001Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)
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117524pEPOR0SP0014Level0 Golden Gate part: CDS, SpCas9-KA
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117525pEPOR0SP0015Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)
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117526pEPOR0SP0016Level0 Golden Gate part: CDS, Sp-eCas9 1.0
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117527pEPOR0SP0017Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)
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117528pEPOR0SP0018Level0 Golden Gate part: CDS, Sp-eCas9 1.1
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117529pEPOR0SP0019Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)
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117530pEPOR0SP0011Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)
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117531pEPOR0SP0020Level0 Golden Gate part: CDS, SaCas9
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117532pEPOR0SP0021Level0 Golden Gate part: CDS, SaCas9 (no stop codon)
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117533pEPOR0SP0012Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)
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117534pEPOR0SP0007Level0 Golden Gate part: CDS, LbCas12a
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117535pEPOR0SP0008Level0 Golden Gate part: CDS, AsCas12a
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117536pICSL50005Level0 Golden Gate part: c-terminal tag, YFP
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117537pEPOR1CB0022Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana
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117538pEPOR1CB0023Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana
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117539pEPOR1CB0058Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana
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117540pEPOR1CB0028Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana
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117541pEPOR1CB0029Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana
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117542pICSL11023Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
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117543pEPOR1CB0002Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
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117544pEPOR1CB0008Level 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plants
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117545pEPOR1CB0009Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants
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117546pEPOR1CB0010Level 1 Golden Gate Cassette: eCas9 1.0 expression cassette for dicotyledonous plants
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117547pEPOR1CB0011Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants
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117548pEPOR1CB0112Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants
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117549pEPOR1CB0013Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants
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117550pEPOR1CB0014Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
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117551pEPOR1CB0015Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants
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117552pEPOR1CB0113Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants
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117553pEPOR1CB0114Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana
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117554pEPOR1CB0018Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
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117555pEPOR1CB0019Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
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117556pEPOR1CB0020Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
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117557pEPOR1CB0021Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
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117558pEPOR1CB0027Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtADH gene gene in A. thaliana with 1 mismatch at PAM
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117559pEPOR1CB0071Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting RPS4A family protein {AT5G58420 } in A. thaliana
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117560pEPOR1CB0075Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Rpn2/Psmd1 subunit {AT2G32730} in A. thaliana
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117561pEPOR1CB0081Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtMPK19 {AT3G14720} in A. thaliana
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117562pEPOR1CB0083Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Methyltransferase {AT5G10620} in A. thaliana
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117563pEPOR1CB0084Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCPK34 {AT5G19360} in A. thaliana
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117564pEPOR1CB0087Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtUUAT1 {AT5G04160} in A. thaliana
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117565pEPOR1CB0089 Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting {AT1G68250} in A. thaliana
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117566pEPOR1CB0092Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCas6 {AT5G04770} in A. thaliana
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117567pEPOR1CB0103Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCas6 {AT5G04770} in A. thaliana
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117568pEPOR1CB0093Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana
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117569pEPOR1CB0104Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana
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117570pEPOR1CB0098Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thaliana
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117571pEPOR1CB0109Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thaliana
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117572pEPOR1CB0099Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana
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117573pEPOR1CB0110Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana
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117574pEPOR1CB0100Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting ENTH/ANT {AT1G14910} in A. thaliana
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117575pEPOR1CB0111Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting ENTH/ANT {AT2G01600} in A. thaliana
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117576pEPOR2KN0001Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)
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117577pEPOR2KN0002Level2 MoClo construct, containing level1 plant expression cassettes for SpCas-p(pEPOR1CB0002) and sgRNA_NbPDS(pEPOR1CB0114)
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117578pEPOR2KN0003Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)
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117579pEPOR2KN0004Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)
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117580pEPOR2KN0008Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)
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117581pEPOR2KN0009Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)
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117582pEPOR2KN0010Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)
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117583pEPOR2KN0011Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)
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117584pEPOR2KN0012Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)
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117585pEPOR2KN0013Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)
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117586pEPOR2KN0014Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)
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117587pEPOR2KN0015Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)
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117588pEPOR2KN0016Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)
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117589pEPOR2KN0017Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS(pEPOR1CB0114)
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117590pEPOR2KN0018Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)
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117591pEPOR2KN0019Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)
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117592pEPOR2KN0020Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)
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117593pEPOR2KN0021Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)
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117594pEPOR2KN0022Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS(pEPOR1CB0114)
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117595pEPOR2KN0023Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)
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117596pEPOR2KN0024Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)
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117597pEPOR2KN0025Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)
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117598pEPOR2KN0026Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)
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117599pEPOR2KN0027Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS(pEPOR1CB0114)
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117600pEPOR2KN0028Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)
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117601pEPOR2KN0029Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)
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117602pEPOR2KN0030Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)
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117603pEPOR2KN0031Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)
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117604pEPOR2KN0032Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0027)
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117605pEPOR2KN0038Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)
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117606pEPOR2KN0039Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)
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117607pEPOR2KN0040Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)
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117608pEPOR2KN0041Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071)
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117609pEPOR2KN0042Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071)
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117610pEPOR2KN0043Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071)
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117611pEPOR2KN0048Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)
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117612pEPOR2KN0045Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)
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117613pEPOR2KN0046Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)
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117614pEPOR2KN0047Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)
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117615pEPOR2KN0044Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071)
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117616pEPOR2KN0049Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
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117617pEPOR2KN0050Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
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117618pEPOR2KN0051Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
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117619pEPOR2KN0052Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
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117620pEPOR2KN0053Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
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117621pEPOR2KN0054Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
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117622pEPOR2KN0055Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
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117623pEPOR2KN0056Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
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117624pEPOR2KN0057Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)
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117625pEPOR2KN0058Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)
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117626pEPOR2KN0059Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)
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117627pEPOR2KN0060Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)
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117628pEPOR2KN0061Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)
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117629pEPOR2KN0062Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)
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117630pEPOR2KN0063Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)
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117631pEPOR2KN0064Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)
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117632pEPOR2KN0065Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)
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117633pEPOR2KN0066Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)
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117634pEPOR2KN0067Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)
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117635pEPOR2KN0068Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)
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117636pEPOR2KN0069Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)
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117637pEPOR2KN0070Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)
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117638pEPOR2KN0071Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
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117639pEPOR2KN0072Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
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117640pEPOR2KN0073Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
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117641pEPOR2KN0074Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
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117642pEPOR2KN0075Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Flavin-binding monooxygenase family protein {AT1G62600}(pEPOR1CB0098)and sgRNA_Flavin-binding monooxygenase family protein {AT1G62590}(pEPOR1CB0109)
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117643pEPOR2KN0076Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)
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117644pEPOR2KN0077Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110)
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117645pEPOR2KN0078Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
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117646pEPOR2KN0079Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
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117647pEPOR2KN0080Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
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