PurposeLevel 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117550||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
- Promoter 2x35S+5'UTR OMEGA (pICH51288)
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: AsCas12a (with stop codon)+3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEPOR1CB0014 was a gift from Nicola Patron (Addgene plasmid # 117550 ; http://n2t.net/addgene:117550 ; RRID:Addgene_117550)
For your References section:Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. Raitskin O, Schudoma C, West A, Patron NJ. PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. 10.1371/journal.pone.0211598 PubMed 30811422