GfaABC1D BARK D110A p2A mCherry
(Plasmid #117692)

• Purpose: GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing
• Depositing Lab: Baljit Khakh
• Publication: Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9. doi: 10.1016/j.neuron.2021.05.023. Epub 2021 Jun 16.

Backbone

• Vector backbone: pZac2.1
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BARKrgs D110A peptide
• Alt name: GfaABC1D-iBARK(D110A)-p2A-mCherry
• Species: B. taurus (bovine)
• Insert Size (bp): 1254
• Mutation: D110A
• Tag / Fusion Protein:
  • HA / FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pZac2.1_gfaABC1D BARK (D110A) p2A mCherry WPRE SV40.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry