pCINeo-mCHMP5
(Plasmid
#11771)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCI-Neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechromatin modifying protein 5
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Alt nameCHMP5
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SpeciesM. musculus (mouse)
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Insert Size (bp)660
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GenBank IDBC006947
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Entrez GeneChmp5 (a.k.a. 2210412K09Rik, AW545668)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCINeo-mCHMP5 was a gift from Sankar Ghosh (Addgene plasmid # 11771 ; http://n2t.net/addgene:11771 ; RRID:Addgene_11771) -
For your References section:
CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis. Shim JH, Xiao C, Hayden MS, Lee KY, Trombetta ES, Pypaert M, Nara A, Yoshimori T, Wilm B, Erdjument-Bromage H, Tempst P, Hogan BL, Mellman I, Ghosh S. J Cell Biol. 2006 Mar 27. 172(7):1045-56. 10.1083/jcb.200509041 PubMed 16567502