pMX-Flag-WIP
(Plasmid #11772)

• Depositing Lab: Jack Strominger
• Publication: Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Backbone

• Vector backbone: pMX-IRES-GFP
• Backbone manufacturer: Liu et al (see Addgene's vector database for details)
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WIP
• Alt name: WASp Interacting Protein
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• GenBank ID: BC002914
• Entrez Gene: WIPF1 (a.k.a. PRPL-2, WAS2, WASPIP, WIP)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Raif Geha (Boston Children's Hospital) provided the wild-type WIP gene, which we cloned into pMX-IRES-GFP.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry