|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117818||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKarl J. Clark
- Backbone size w/o insert (bp) 2431
Vector typeSynthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1398
/ Fusion Protein
- CAAX (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer GCATGGATGTTTTCCCAGTC
- 3′ sequencing primer atggctcataacaccccttg (Common Sequencing Primers)
Terms and Licenses
Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Control-cx43.4-48H-pGTag was a gift from Jeffrey Essner (Addgene plasmid # 117818 ; http://n2t.net/addgene:117818 ; RRID:Addgene_117818)
For your References section:GeneWeld: a method for efficient targeted integration directed by short homology. Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Weiss TJ, Torrie ME, Vollbrecht MK, Lan M, McKeighan KC, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity B, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner JJ.. bioRxiv 431627 10.1101/431627