tetO-Bcl2-IRES-tdtomato(TET005)
(Plasmid
#117857)
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PurposeThis plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.
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Depositing Lab
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Depositing OrganizationStowers Institute for Medical Research
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 117857-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 117857-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonetetO-Kir2.1-IRES-tauLacZ
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Backbone manufacturerYu et al.
- Backbone size w/o insert (bp) 6044
- Total vector size (bp) 6755
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Modifications to backbonetau-LacZ was replaced with tdTomato.
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Vector typeMammalian Expression ; tTA/tetO
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl2
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Alt nameB-cell lymphoma 2
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SpeciesM. musculus (mouse)
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Insert Size (bp)711
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Entrez GeneBcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
- Promoter tetO
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTAAAACGACGGCCAG
- 3′ sequencing primer CAGGAAACAGCTATGAC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 117857-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 117857-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO-Bcl2-IRES-tdtomato(TET005) was a gift from C. Ron Yu (Addgene plasmid # 117857 ; http://n2t.net/addgene:117857 ; RRID:Addgene_117857) -
For your References section:
A Population of Navigator Neurons Is Essential for Olfactory Map Formation during the Critical Period. Wu Y, Ma L, Duyck K, Long CC, Moran A, Scheerer H, Blanck J, Peak A, Box A, Perera A, Yu CR. Neuron. 2018 Dec 5;100(5):1066-1082.e6. doi: 10.1016/j.neuron.2018.09.051. Epub 2018 Oct 25. 10.1016/j.neuron.2018.09.051 PubMed 30482691
Map uploaded by the depositor.
