Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

tetO-Bcl2-IRES-tdtomato(TET005)
(Plasmid #117857)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117857 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    tetO-Kir2.1-IRES-tauLacZ
  • Backbone manufacturer
    Yu et al.
  • Backbone size w/o insert (bp) 6044
  • Total vector size (bp) 6755
  • Modifications to backbone
    tau-LacZ was replaced with tdTomato.
  • Vector type
    Mammalian Expression ; tTA/tetO

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl2
  • Alt name
    B-cell lymphoma 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    711
  • Entrez Gene
    Bcl2 (a.k.a. AW986256, Bcl-, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
  • Promoter tetO

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAG
  • 3′ sequencing primer CAGGAAACAGCTATGAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO-Bcl2-IRES-tdtomato(TET005) was a gift from C. Ron Yu (Addgene plasmid # 117857 ; http://n2t.net/addgene:117857 ; RRID:Addgene_117857)