CMV-mGFP-pA(AV222)
(Plasmid
#117858)
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PurposeThis plasmid expresses membrane anchored GFP to facility axon morphology study.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAdeasy-1
- Backbone size w/o insert (bp) 33339
- Total vector size (bp) 34901
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGFP
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Alt namemembrane GFP
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Alt namemembrane anchored green fluorescent protein
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SpeciesSynthetic
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Insert Size (bp)843
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CACAATGCTTCCATCAAACG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-mGFP-pA(AV222) was a gift from C. Ron Yu (Addgene plasmid # 117858 ; http://n2t.net/addgene:117858 ; RRID:Addgene_117858) -
For your References section:
A Population of Navigator Neurons Is Essential for Olfactory Map Formation during the Critical Period. Wu Y, Ma L, Duyck K, Long CC, Moran A, Scheerer H, Blanck J, Peak A, Box A, Perera A, Yu CR. Neuron. 2018 Dec 5;100(5):1066-1082.e6. doi: 10.1016/j.neuron.2018.09.051. Epub 2018 Oct 25. 10.1016/j.neuron.2018.09.051 PubMed 30482691
