PurposeAAV vector for sparse and bright labeling in a cell-type specific manner
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118029||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
MutationSee depositor comments below
- Promoter TRE
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Note: vCre contains an A347T mutation. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-TRE-dDIO-vCre was a gift from Minmin Luo (Addgene plasmid # 118029 ; http://n2t.net/addgene:118029 ; RRID:Addgene_118029)
For your References section:Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M. Nat Methods. 2018 Nov 19. pii: 10.1038/s41592-018-0184-y. doi: 10.1038/s41592-018-0184-y. 10.1038/s41592-018-0184-y PubMed 30455464