pMST-713
(Plasmid
#118081)
-
Purposeptet-on::cexA::ttrpC
-
Depositing Lab
-
Depositing OrganizationUniversitaet fur Bodenkultur Wien (BOKU-WIEN)
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 118081-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 118081-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepMST635, pMST1212
-
Backbone manufacturerSarkari et al. 2017
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCexA
-
SpeciesA. niger
-
Insert Size (bp)1575
- Promoter ptet-on
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGGTCACCCCTAGAGACAGTGA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 118081-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 118081-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMST-713 was a gift from Michael Sauer (Addgene plasmid # 118081 ; http://n2t.net/addgene:118081 ; RRID:Addgene_118081) -
For your References section:
Engineering of the citrate exporter protein enables high citric acid production in Aspergillus niger. Steiger MG, Rassinger A, Mattanovich D, Sauer M. Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13. 10.1016/j.ymben.2018.12.004 PubMed 30553933
Map uploaded by the depositor.
