pET23b-Casp2-Delta Card
(Plasmid #11810)

• Depositing Lab: Guy Salvesen
• Publication: Salvesen Casp1, Casp2 plasmids (unpublished)

Backbone

• Vector backbone: pET-23 b
• Backbone size w/o insert (bp): 3666
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase-2 Delta Card
• Alt name: Caspase-2
• Species: H. sapiens (human)
• Insert Size (bp): 943
• Mutation: Delta Card
• Entrez Gene: CASP2 (a.k.a. CASP-2, ICH1, MRT80, NEDD-2, NEDD2, PPP1R57)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: T7 terminal primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry