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pTXB1-xlH2B(1-114)_K114A
(Plasmid #118217)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118217 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTXB1
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6706
  • Total vector size (bp) 6998
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    xlH2B(1-114)
  • Alt name
    Histone H2B N-terminal fragment
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    342
  • Mutation
    Lysine 114 to alanine
  • GenBank ID
    AJ556871.1
  • Promoter T7
  • Tag / Fusion Protein
    • Mxe-GyrA intein fused to chitin binding domain (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXB1-xlH2B(1-114)_K114A was a gift from Cynthia Wolberger (Addgene plasmid # 118217 ; http://n2t.net/addgene:118217 ; RRID:Addgene_118217)
  • For your References section:

    Structural basis for histone H2B deubiquitination by the SAGA DUB module. Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C. Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681. 10.1126/science.aac5681 PubMed 26912860