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Structural basis for histone H2B deubiquitination by the SAGA DUB module.
Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C
Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
75299His6TEV-UbG76CBacteria expression plasmid for Ubiquitin G76C with removalbe His6 tag by TEV protease
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75332H2BK120CBacteria expression of H2BK120C
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118217pTXB1-xlH2B(1-114)_K114ABacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
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118252pTXB1-H2B(1-114)_wtBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
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