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Flag-Fos S374A
(Plasmid #118302)


Item Catalog # Description Quantity Price (USD)
Plasmid 118302 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    C-Fos S374A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    Fos (a.k.a. D12Rfj1, c-fos, cFos)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

site directed mutagenesis. Fos constructs were subcloned into pcDNA3 containing Flag epitope with EcoRI and XbaI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Fos S374A was a gift from Philip Stork (Addgene plasmid # 118302 ; ; RRID:Addgene_118302)
  • For your References section:

    Sustained activation of extracellular signal-regulated kinase by nerve growth factor regulates c-fos protein stabilization and transactivation in PC12 cells. Pellegrino MJ, Stork PJ. J Neurochem. 2006 Dec;99(6):1480-93. doi: 10.1111/j.1471-4159.2006.04250.x. 10.1111/j.1471-4159.2006.04250.x PubMed 17223854