GFP-Epac2
(Plasmid #118314)

• Purpose: Expression of mouse Epac2
• Depositing Lab: Philip Stork
• Publication: Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25. doi: 10.1128/MCB.01060-08. Epub 2008 Sep 29.

Backbone

• Vector backbone: peGFP-C1
• Backbone manufacturer: clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Epac2
• Species: M. musculus (mouse)
• Insert Size (bp): 2982
• Entrez Gene: Rapgef4 (a.k.a. 1300003D15Rik, 5730402K07Rik, 6330581N18Rik, EPAC 2, Epac-2, Epac2, mKIAA4040)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (unknown if destroyed)
• 3′ cloning site: blunted NotI/SmaI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HA-mouse Epac2 was obtained from Lawrence Quilliam (Indiana University)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry