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GFP-Epac2
(Plasmid #118314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118314 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 118314-D50 50 μg of DNA in Tris buffer $495
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DNA 118314-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epac2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2982
  • Entrez Gene
    Rapgef4 (a.k.a. 1300003D15Rik, 5730402K07Rik, 6330581N18Rik, EPAC 2, Epac-2, Epac2, mKIAA4040)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site blunted NotI/SmaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HA-mouse Epac2 was obtained from Lawrence Quilliam (Indiana University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Epac2 was a gift from Philip Stork (Addgene plasmid # 118314 ; http://n2t.net/addgene:118314 ; RRID:Addgene_118314)

  • For your References section:

    Ras is required for the cyclic AMP-dependent activation of Rap1 via Epac2. Liu C, Takahashi M, Li Y, Song S, Dillon TJ, Shinde U, Stork PJ. Mol Cell Biol. 2008 Dec;28(23):7109-25. doi: 10.1128/MCB.01060-08. Epub 2008 Sep 29. 10.1128/MCB.01060-08 PubMed 18824540
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